Miniaturized modular-array fluorescence microscopy

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Programmable Array Fluorescence Lifetime Spectroscopy/microscopy Using Tcspc

Spatial light modulators (SLMs) are a family of optical devices that allow modulation of intensity and/or phase in light microscopy to control excitation and emission. There are broadly two classes of devices employed: digital micromirror arrays (DMDs) and ferroelectric liquid crystal devices. DMDs have found use in controlled light exposure microscopy (CLEM) to reduce photobleaching and photot...

متن کامل

Gigapixel fluorescence microscopy with a water immersion microlens array.

We demonstrate high throughput gigapixel fluorescence microscopy with a microlens array. We show, for the first time to the best of our knowledge, the use of a parallelized microscopy system to image samples in micro well plates. We image centimeter-scale regions of 384-well micro well plates at 1.72 μm resolution at a raw pixel throughput of 25.4 Mpx/s. Taking into account the fact that about ...

متن کامل

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

In vivo circuit and cellular level functional imaging is a critical tool for understanding the brain in action. High resolution imaging of mouse cortical neurons with two-photon microscopy has provided unique insights into cortical structure, function and plasticity. However, these studies are limited to head fixed animals, greatly reducing the behavioral complexity available for study. In this...

متن کامل

Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging.

Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-s...

متن کامل

Fluorescence and fluorescence microscopy

The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biomedical Optics Express

سال: 2020

ISSN: 2156-7085,2156-7085

DOI: 10.1364/boe.410605